Composite
Part:BBa_K606022:Design
Designed by: Kvin Yauy Group: iGEM11_Paris_Bettencourt (2011-07-27)
pVeg-SpoVG-GFP-lacI-Term for B. Subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 856
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 767
Design Notes
Switch from pSB1AK3 plasmid to pSB1C3
Source
GFP:LacI comes from Andrew Wright, Dave Lane, pSG20 plasmid.